Superresolution live imaging of plant cells using structured illumination microscopy. in Quantitative Imaging in Cell Biology 123, 295–313 (Elsevier, 2014).
Practical structured illumination microscopy. Structured illumination microscopy for superresolution. in Fluorescence Microscopy 213–225 (Elsevier, 2014).Īllen, J.R., Ross, S.T. Response to comment on 'Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics'. Comment on 'Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics'.
Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating. The calibration sample preparation and system calibration protocol can be executed within 1–2 d.
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This protocol, plus accompanying guidelines, allows researchers from students to imaging professionals to create an optimal SIM imaging environment regardless of specimen type or structure of interest. It also includes a conceptual overview and checklist for experimental design and calibration decisions, and is applicable to any commercially available or custom platform. The protocol details preparation of calibration bead slides designed for SIM-based experiments, the acquisition of calibration data, the documentation of typically encountered SIM artifacts and corrective measures that should be taken to reduce them. Here we present a protocol that allows users to generate high-quality SIM data while accounting and correcting for common artifacts. However, the reliance of SIM on algorithmic post-processing renders it particularly sensitive to artifacts that may reduce resolution, compromise data and its interpretations, and drain resources in terms of money and time spent.
Linear 2D- or 3D-structured illumination microscopy (SIM or3D-SIM, respectively) enables multicolor volumetric imaging of fixed and live specimens with subdiffraction resolution in all spatial dimensions.
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